CircRNA CRDMO

在線訂購

產品介紹
案例分析

circRNA CRDMO Service

Circular RNA, with greater stability attributed to its unique closed-loop structure, is an ideal platform for the development of next-generation gene therapy as an effective alternative to linear mRNA. Geneseed has been focusing on circRNA for more than 10 years and has profound technology accumulation. Geneseed has two patented RNA circularization strategies: Group Ⅰintron self-splicing and T4 Rnl2 method. We have established platforms for sequence design, process development, analytical method development, scale production, QC, raw material preparation, etc., which can provide biopharma with CRDMO services in the whole process from early R&D to commercial production.

Our Serveice

Manufacturing Process from Plasmid to circRNA

Quality Analysis of circRNA

Quality Analysis of circRNA-LNP

Advantages

1. Two independent intellectual property rights, comprehensively matching the needs of the project：circPrecise? by Group I intron self-splicing，circPure? by T4 Rnl2；

2. Unique sequence design approach，circularization efficiency＞90%：using modular translation element screening and secondary structure prediction of IRES and CDS interaction, the translation efficiency is greatly improved；

3. Robust amplification production process，mass production scale up：flexible IVT- circularization scale from μg to g grade for different templates；

4. Various purification strategies，purity＞95%：analyze impurity components and concentration to customize a personalized chromatographic purification strategy；

5. Common LNP encapsulation platform，efficiency＞95%：self-developed liposome screening, platform for different formulations；

6. Multi-dimensional QC platform：develop analytical methods to match the needs of project, and meet the QC evaluation of each stage;

7. Efficient raw material synthesis platform to reduce production costs significantly.

Catalog CircRNA Products

Except for circ-EPO, circ-Trp53, circ-TP53, circ-eGFP, circ-Fluc, circ-mCherry and Natural Circular RNA, 3xFlag labels were inserted to other Catalog CircRNA Products, and anti-Flag antibodies can be used to detect their protein products.

Product Category	Name	Model	Specification
Reporter gene	GSPure? Circ-eGFP	R0701 / R0702 / R0703 / R0704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-Fluc	R0801 / R0802 / R0803 / R0804	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-mCherry	R0901 / R0902 / R0903 / R0904	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-beta gal	R1401 / R1402 / R1403 / R1404	100 μg / 500 μg / 1 mg / 5 mg
Tumor antigen	GSPure? Circ-OVA	R1501 / R1502 / R1503 / R1504	100 μg / 500 μg / 1 mg / 5 mg
Anti-tumor	GSPure? Circ-TP53	R1701 / R1702 / R1703 / R1704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-Trp53	R2501 / R2502 / R2503 / R2504	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-PTEN-L (homo)	R1001 / R1002 / R1003 / R1004	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-PTEN-L (mmu)	R1101 / R1102 / R1103 / R1104	100 μg / 500 μg / 1 mg / 5 mg
Growth factor	GSPure? Circ-VEGFA165 (homo)	R1201 / R1202 / R1203 / R1204	100 μg / 500 μg / 1 mg / 5 mg
Growth factor	GSPure? Circ-VEGFA165 (mmu)	R1301 / R1302 / R1303 / R1304	100 μg / 500 μg / 1 mg / 5 mg
immunotherapy	GSPure? Circ-IL2 (homo)	R1901 / R1902 / R1903 / R1904	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-IL2 (mmu)	R3201 / R3202 / R3203 / R3204	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-IL12a	R3301 / R3302 / R3303 / R3304	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-IL12A	R3401 / R3402 / R3403 / R3404	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-IL12B	R3501 / R3502 / R3503 / R3504	100 μg / 500 μg / 1 mg / 5 mg
Gene editing	GSPure? Circ-Cas9	R2001 / R2002 / R2003 / R2004	100 μg / 500 μg / 1 mg / 5 mg
Protein/ Antibody replacement	GSPure? Circ-EPO (homo)	R2101 / R2102 / R2103 / R2104	100 μg / 500 μg / 1 mg / 5 mg
Protein/ Antibody replacement	GSPure? Circ-EPO (mmu)	R2301 / R2302 / R2303 / R2304	100 μg / 500 μg / 1 mg / 5 mg
iPSC reprogramming	GSPure? Circ-SOX2 (homo)	R2201 / R2202 / R2203 / R2204	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-SOX2 (mmu)	R2401 / R2402 / R2403 / R2404	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-OCT4 (homo)	R2601 / R2602 / R2603 / R2604	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-OCT4 (mmu)	R2701 / R2702 / R2703 / R2704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-KLF4 (homo)	R2801 / R2802 / R2803 / R2804	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-KLF4 (mmu)	R2901 / R2902 / R2903 / R2904	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-C-Myc (homo)	R3001 / R3002 / R3003 / R3004	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-C-Myc (mmu)	R3101 / R3102 / R3103 / R3104	100 μg / 500 μg / 1 mg / 5 mg
Anti-aging	GSPure? Circ-TERT	R3601 / R3602 / R3603 / R3604	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-Tert	R3701 / R3702 / R3703 / R3704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FGF21 (homo)	R3801 / R3802 / R3803 / R3804	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FGF21 (mmu)	R3901 / R3902 / R3903 / R3904	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-αKlotho (homo)	R4001 / R4002 / R4003 / R4004	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-αKlotho (mmu)	R4101 / R4102 / R4103 / R4104	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-sTGFβR2 (homo)	R4201 / R4202 / R4203 / R4204	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-sTGFβR2 (mmu)	R4301 / R4302 / R4303 / R4304	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FGF23 (homo)	R4401 / R4402 / R4403 / R4404	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FGF23 (mmu)	R4501 / R4502 / R4503 / R4504	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FGF17 (homo)	R4601 / R4602 / R4603 / R4604	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FGF17 (mmu)	R4701 / R4702 / R4703 / R4704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-SOX8 (homo)	R4801 / R4802 / R4803 / R4804	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-SOX8 (mmu)	R4901 / R4902 / R4903 / R4904	100 μg / 500 μg / 1 mg / 5 mg
Neurotrophic factor	GSPure? Circ-NGF (homo)	R5001 / R5002 / R5003 / R5004	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NGF (mmu)	R5101 / R5102 / R5103 / R5104	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-BDNF (homo)	R5201 / R5202 / R5203 / R5204	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-BDNF (mmu)	R5301 / R5302 / R5303 / R5304	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-GDNF (homo)	R5401 / R5402 / R5403 / R5404	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-GDNF (mmu)	R5501 / R5502 / R5503 / R5504	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NT3 (homo)	R5601 / R5602 / R5603 / R5604	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NT3 (mmu)	R5701 / R5702 / R5703 / R5704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NT4 (homo)	R5801 / R5802 / R5803 / R5804	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NT4 (mmu)	R5901 / R5902 / R5903 / R5904	100 μg / 500 μg / 1 mg / 5 mg
Nerve regeneration transforming factor	GSPure? Circ-NeuroD1 (homo)	R6001 / R6002 / R6003 / R6004	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NeuroD1 (mmu)	R6101 / R6102 / R6103 / R6104	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NeuroD2 (homo)	R6201 / R6202 / R6203 / R6204	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NeuroD2 (mmu)	R6301 / R6302 / R6303 / R6304	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NeuroD6 (homo)	R6401 / R6402 / R6403 / R6404	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NeuroD6 (mmu)	R6501 / R6502 / R6503 / R6504	100 μg / 500 μg / 1 mg / 5 mg
Natural circular RNA	GSPure? Circ-PVT1 (homo)	R6601 / R6602 / R6603 / R6604	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-PVT1 (mmu)	R6701 / R6702 / R6703 / R6704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FOXO3 (homo)	R6801 / R6802 / R6803 / R6804	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-FOXO3 (mmu)	R6901 / R6902 / R6903 / R6904	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-HIPK3	R7001 / R7002 / R7003 / R7004	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-ZNF609	R7101 / R7102 / R7103 / R7104	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-ZFR	R7201 / R7202 / R7203 / R7204	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-SMARCA5	R7301 / R7302 / R7303 / R7304	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-PRKCI	R7401 / R7402 / R7403 / R7404	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-NRIP1	R7501 / R7502 / R7503 / R7504	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-ABCB10	R7601 / R7602 / R7603 / R7604	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-CDYL	R7701 / R7702 / R7703 / R7704	100 μg / 500 μg / 1 mg / 5 mg
	GSPure? Circ-Mbl	R7801 / R7802 / R7803 / R7804	100 μg / 500 μg / 1 mg / 5 mg

High integrity

Figure 1: GSPure? Circ-eGFP (left) and GSPure? Circ-mCherry (right) integrity (CE) > 95% after purification

High purity

Figure 2: GSPure? Circ-eGFP (left) and GSPure? Circ-Fluc (right) purity (HPLC) > 95% after purification

High quality

Fig 3: Fluorescence detection results after GSPure?Circ-eGFP transfected 293T cells, magnification: 100×.

Fig 4: Fluorescence detection results after GSPure? Circ-mCherry transfected 293T cells, magnification: 100×.

Fig 5: Results of luciferase detection after GSPure? Circ-Fluc transfection of 293T cells.